ELISA: Theory and Practice

摘要: Basic Immunology: 1. The Immune Response 2. Antigens 3. Adaptive Immunity and Clonal Selection 4. Antibodies 5. Antigenic Commonness Principles of ELISA: Reaction Schemes Direct ELISA Indirect Sandwich Competition 6. Choice Assays Stages in Solid-Phase Washing Addition Reagents Incubation Blocking Conditions Nonspecific Reactions Enzyme Conjugates 7. Availability 8. Conjugation with Enzymes 9. Development Label 10. Stopping 11. Reading 12. Practical Problems Theoretical Considerations: Setting Up Use What Is Already Known Complexity Considerations Other Techniques Units Making Dilutions Pipets Molarities Titration Antigen Antibody: Learning Scheme Basis Assay Materials Equipment Details Data Explained Aspects Described Conclusions to Titrate Determine the Positivity Sera at Single Dilution on Capture Detect Methods Antibody Using IgG when Used as Whole Serum Competitive General Information for Detection Quantification Assay-Antigen Antibody-Detection a Test Overall Immunochemical Techniques: Introduction Labeling Coupling Alkaline Phosphatase Preparation Immunoglobulins Immunosorbents Production Antisera