A New Approach of Sperm Motility Subpopulation Structure in Donkey and Horse

摘要: This study aimed to characterize the sperm kinematic values with high frames per second, in order define subpopulation structure of horse and donkey compare them. A total 51 fresh semen ejaculates (26 Spanish 16 Arabian breeds, 12 donkeys) were collected, subsequently analyzed for parameters by using computer-assisted analysis (CASA‐Mot) ISAS®v1.2 system a Spermtrack® 10µm depth counting chamber. Sequences recorded at 250 second eight automatically evaluated. All showed significant differences between horse, breeds. evaluated excellent motility characteristics; significantly higher all donkeys compared horses except BCF. Donkey was faster linear than horse. Regarding breeds differences, had VAP, VCL BCF rapid but Arab more linear. The principal component three subpopulations ejaculate different characteristic dominant both rapid, straight beat (38.2% 41.7% respectively) while lowest slowest non-linear sperms. This, plus slight distribution these found. In conclusion, permitted have new interpretation motile specie breed differences. More so, future works on spermatozoa should take into account which may interfere alter real performed.