Magnetic resonance spectroscopy to detect functional improvement by adipose tissue-derived stromal cell transplantation in the ischemic hind limb in diabetic rats

摘要: Adipose tissue-derived stromal cells (ADSCs) are a prominent cellular source in regenerative medicine. We tested whether transplantation of ADSCs into ischemic muscular tissue diabetic animals would attenuate the impairment cell metabolism and microcirculatory with combination techniques including magnetic resonance imaging spectroscopy (1H-MRS). Methods: induced unilateral hind limb ischemia male streptozotocin-treated rats non-diabetic controls. One day after femoral artery ligation, 6 per group were randomly injected intramuscularly allogeneic (C1: 106, C2: 107, C3: 108 cells/mL); or conditioned media from ADSC cultures (CM); saline (control). Rats underwent angiography (MRA); short time inversion recovery (STIR) edema-weighed imaging; 1H-MRS; immunoblotting immunofluorescence on both limbs for 4 weeks. Results: T1-weighted STIR images showed presence swelling signal hyperintensity respectively affected by occlusion. Mean total ratio creatine/water (tCr/water) occluded was significantly lower than non-occluded rats. At weeks, CM groups had greater tCr/water compared controls diabetic- (**p<0.01; *p<0.05) (Figure 1, panel A), less (panel B), increased expression α-sarcomeric actinin, vascular endothelial growth factor (VEGF) hepatocyte (HGF) (Table 1), as well vessel density C) suppression up-regulation water channel aquaporin (AQP)-1 pro-inflammatory early response gene such cycloxygenase (COX)-2 D). ![Figure][1] Figure 1 View this table: Table 1. VEGF HGF rat tissues ligation treatments medium controls Conclusions: improve muscle increase neovasculogenesis ischemia. 1H-MRS is useful tool to monitor attempts at salvaging cell-derived novel therapies. [1]: pending:yes