Volatilization of high molecular weight DNA by pulsed laser ablation of frozen aqueous solutions

作者: R. Nelson , M. Rainbow , D. Lohr , P Williams

DOI: 10.1126/SCIENCE.2595370

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摘要: DNA has been volatilized by pulsed laser ablation of a thin film frozen aqueous solution. The target was irradiated in vacuum at power densities sufficient to ablate the ice matrix. expanding ablated water vapor entrained embedded molecules and expelled them into gas phase. Ejection as large 410 kilodaltons verified collection products subsequent mass analysis polyacrylamide gel electrophoresis with autoradiographic detection.

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