Chapter XII Reflectance Spectrophotometry

摘要: Publisher Summary The separation and determination of an absorbing substance from microbial material is often impracticable. A measure concentration may be obtained reflectance spectrophotometry using a procedure analogous to that the analysis transparent media by transmission spectrophotometry. When bands are widely separated, evaluation band intensity, width shape relatively simple matter. absorption spectra biological materials exhibit overlapping bands, measurement characteristics cannot made separately for each band. An analytical technique minimizes this difficulty has been used particularly estimation cytochrome in micro-organisms. In medical field, changes tissue chemistry produced disease affects differences infrared attenuated total tissues.