摘要: In the previous chapter we presented an overview of protein fluorescence. We described spectral properties aromatic amino acids and how these depend on structure. now extend this discussion to include time-resolved measurements intrinsic Prior 1983, most fluorescence were performed using TCSPC. The instruments employed for typically used a flashlamp excitation source standard dynodechain-type PMT. Such provided instrument response functions with half-width near 2 ns, which is comparable decay time proteins. limited repetition rate flashlamps, 20 kHz, resulted in data modest statistical accuracy, unless acquisition times excessively long. Given complexity intensity anisotropy decays, inherent difficulty resolving multiexponential processes, it was difficult obtain definitive information kinetics
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