Functional csdA is needed for effective adaptation and initiation of growth of Clostridium botulinum ATCC 3502 at suboptimal temperature.

摘要: Abstract The activity of RNA helicase csdA ( cbo2802 ) after temperature downshift was compared to its at optimal growth temperature, and the effect sense antisense oriented insertional inactivation on ATCC 3502 suboptimal evaluated. relative transcript level significantly induced for 30 min 5 h cold shock. In contrast, a significant decrease in observed within same time frame 37 °C. Inactivation led an extensive delay initiation exponential 20 °C but not addition, mean minimum temperatures mutant strains were higher than those wild-type strain. During 24-hour incubation 37 °C, all motile, whereas showed severely impaired motility This study shows that functional is needed effective adaptation Clostridium botulinum temperature.