Pmr, a Histone-Like Protein H1 (H-NS) Family Protein Encoded by the IncP-7 Plasmid pCAR1, Is a Key Global Regulator That Alters Host Function

摘要: Histone-like protein H1 (H-NS) family proteins are nucleoid-associated (NAPs) conserved among many bacterial species. The IncP-7 plasmid pCAR1 is transmissible various Pseudomonas strains and carries a gene encoding the H-NS protein, Pmr. putida KT2440 host of pCAR1, which harbors five genes PP_1366 (TurA), PP_3765 (TurB), PP_0017 (TurC), PP_3693 (TurD), PP_2947 (TurE). Quantitative reverse transcription-PCR (qRT-PCR) demonstrated that presence does not affect transcription these only pmr, turA, turB were primarily transcribed in KT2440(pCAR1). In vitro pull-down assays revealed Pmr strongly interacted with itself TurA, TurB, TurE. Transcriptome comparisons pmr disruptant, KT2440, KT2440(pCAR1) indicated disruption had greater effects on transcriptome than did carriage. transcriptional levels some increased carriage, such as mexEF-oprN efflux pump parI, reverted to pCAR1-free KT2440. Transcriptional putative horizontally acquired altered by carriage but disruption. Identification genome-wide binding sites ChAP-chip (chromatin affinity purification coupled high-density tiling chip) analysis preferentially binds DNA regions. overlapped well location differentially following both chromosome. Our findings indicate key factor optimizing