Analysis of microbial mixtures by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

摘要: Many different laboratories are currently developing mass-spectrometric techniques to analyze and identify microorganisms. However, minimal work has been done with mixtures of bacteria. To demonstrate that microbial could be analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), mixed bacterial cultures were in a double-blind fashion. Nine species our MALDI-MS fingerprint library used generate 50 simulated similar for an initial blind study previously reported (Jarman, K. H.; Cebula, S. T.; Saenz, A. J.; Petersen, C. E.; Valentine, N. B.; Kingsley, M. Wahl, L. Anal. Chem. 2000, 72, 1217−1223). The samples automated data extraction analysis algorithms developed laboratory. components present the sample identified correctly level all but one samples. eliminating closel...