Pharmacological properties of a protease from ficus hispiad linn.

摘要: A sulphydryl plant protease present in the latex of Ficus hispida Linn affects hatematological values mice. The isolated was found to increase clotting time and erythrocyte sedimentation rate while haemoglobin conten, RBC count WBC were decreased a dose dependent manner. Ointment containing 1.0% (w/w) hispidain washable ointment base showed good wound healing property also possesses mild anti inflammatory activity. (Family: Moraceae), is moderate sized tree available India some other tropical countries 1-5 . Apart from uses, different parts are used treatment hacmorrhage nose mouth, diseases blood, boils wounds traditional system medicine 2,3 on injury exudes milky sticky 4. isolation form latex, its purification physico- chemical properties have been reported 6. temperature PH for optimum activity 40ocand 7.0 respectively using casein as substrate. Later, we purified further by sephadex G-200 gel filtration upto 36 folds with yield 5.22% determinined molecular weight G-100 which be around 23,700 daltons (unpublished work). Acute toxicity sample has studied (LD50=1230 mg/kg body mice, intraperitoneal). work deals study effects haematological wounds, effect flammatory conditions. EXPERIMENTAL Materials: crude extract precipitation 40-60% ammonium sulphate followed dialysis iyophilization 6 study. Albino mice either sex weighing between 18-24 albino rats pf 150-200g where purchased M/S B.N Ghosh, Calcutta. Heparin Sodium injection (5000 i.u. per ml, Beparine, Biological E.), Soframycin skin cream (W/W) Framycetin Sulphate in; (Hoechst Marion Roussel itd., Mumbai) Vovaran (Dominion pharmaceuticals pvt. Ltd., Bangalore) market. Water base7 prepared our laboratory. All chemicals E. Merck or Ranbaxy laboratories Ltd analytical grade. Methods: