Purification, characterization, and transcriptional analyses of RNA polymerases from Rhodobacter sphaeroides cells grown chemoheterotrophically and photoheterotrophically.
作者： J W Kansy , S Kaplan
DOI: 10.1016/S0021-9258(18)80064-3
关键词:
摘要: Abstract RNA polymerase was purified from Rhodobacter sphaeroides cells grown both chemoheterotrophically and photoheterotrophically. Both preparations of were comprised five major subunits designated: beta' (160,000 Da), beta (150,000 sigma (93,000 alpha (41,000 a 35,000-Da protein, designated epsilon. All the isolated photoheterotrophically found to be serologically related (beta', beta, sigma, alpha) Escherichia coli; however, only four E. coli subunits. The enzyme had lower specific activity considerably less stable than cells. However, synthesis by dependent upon presence DNA template MgCl2, synthetic inhibited rifampicin. transcriptional activities samples studied using different templates, sizes run-off products compared expected values obtained analysis mRNA's produced in vivo. These results are discussed light sequences contain promoter-like regions as determined vivo studies.
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