The expression of genes related to adipocyte differentiation in pigs.
作者： H. C. Wang , Y. H. Ko , H. J. Mersmann , C. L. Chen , S. T. Ding
关键词:
摘要: The purpose of this study was to detect differential expression genes related adipocyte differentiation in pigs by suppression subtractive hybridization. Adipocytes and stromal vascular cells (a fraction containing preadipocytes) from pig adipose tissue were isolated for mRNA extraction. cDNA preadipocytes subtracted the adipocytes. gene fragments cloned into pGEM-T Easy TA cloning vector. We selected 384 clones sequence determination further analysis. These subjected a screening procedure confirm between 2 cell types. found that at least 36 highly expressed adipocytes compared with preadipocytes. Among these, 6 including novel greatest differences confirmed Northern angiotensin I-converting enzyme (ACE), ataxia-telangiectasia mutated protein (ATM), calpain 1, stearoyl coenzyme A desaturase 1 (SCD1) (P < 0.05). relative abundance ACE, ATM, SCD1, discovered current increased later stages results involved lipid metabolism porcine However, investigation is needed demonstrate specific functions study.
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