A Bioguided Approach for the Screening of Antibacterial Compounds Isolated From the Hydroalcoholic Extract of the Native Brazilian Bee's Propolis Using Mollicutes as a Model.

摘要: Nature is a vast source of medicinal substances, including propolis, which has been extensively investigated. Propolis resinous substance produced by bees from the exudates plants that they collect and modify in their jaws; it rich complex matrix with secondary metabolites diverse botanical origins. The objective this study was to apply an vitro bioguided approach using as model system mollicutes sample propolis Brazilian native bee Melipona quadrifasciata (mandacaia) order identify potential new molecules antimicrobial activity. A crude hydroalcoholic extract obtained submitted liquid-liquid partitioning solvents different polarities, generating four fractions: aqueous, dichloromethane, butanol, ethyl acetate fractions. antimollicute activity assays served basis for fractionation. dichloromethane fraction most promising, exhibiting minimal inhibitory concentration (MIC) 125 μg/mL against Mycoplasma pneumoniae. After purification column liquid chromatography, subfraction presenting MIC 15.6 genitalium highlighted. fractions were also tested Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. Using gas chromatography coupled mass spectrometer (GC-MS), several volatile compounds identified non-polar propolis. However, more purified had no better than original subfraction. Apparently, synergism among its largely responsible antibacterial bee.