Downregulation of inhibitor of apoptosis‑stimulating protein of p53 inhibits proliferation and promotes apoptosis of gastric cancer cells

摘要: Gastric cancer (GC) remains one of the leading causes cancer-associated mortality. Inhibitor apoptosis-stimulating protein p53 (iASPP) is a member inhibitory family. The overexpression iASPP has been detected in several types tumor humans. However, role GC to be elucidated. objectives present study were detect expression and examine potential cell lines. Using reverse transcription-quantitative polymerase chain reaction western blot analyses, it was identified that tissues lines higher compared with adjacent normal gastric mucosa line (GES-1). To cells, inhibited using small interfering (si)RNA against observed significantly downregulated. MTT assays, colony-formation assays flow cytometry, inhibition able inhibit proliferation colony forming ability promote apoptosis cells. cells vivo, which infected iASPP-siRNA or control-siRNA subcutaneously injected into nude mice. It downregulation growth vivo. Thus, may molecular target therapy.