Role of HuR in skeletal myogenesis through coordinate regulation of muscle differentiation genes

摘要: In this report, we investigate the role of RNA-binding protein HuR during skeletal myogenesis. At onset myogenesis in differentiating C2C12 myocytes and vivo regenerating mouse muscle, cytoplasmic abundance increased dramatically, returning to a predominantly nuclear presence upon completion mRNAs encoding key regulators myogenesis-specific transcription (myogenin MyoD) cell cycle withdrawal (p21), bearing AU-rich regions, were found be targets differentiation-dependent manner. Accordingly, mRNA half-lives highest differentiation, declining when differentiation was completed. Importantly, HuR-overexpressing cells displayed target expression half-life underwent precocious differentiation. Our findings underscore critical function for linked HuR's coordinate regulation muscle genes.