Translational regulation of human p53 gene expression.

摘要: In blast cells obtained from patients with acute myelogenous leukemia, p53 mRNA was present in all the samples examined while expression of protein variable patient to patient. Mutations gene are infrequent this disease and, hence, majority cannot be accounted for by mutation. study, we regulation human leukemic blasts and characterized transcripts these cells. We found control both at level RNA abundance translation. Four experiments point towards translational expression. First, there is no correlation between Second, two cell lines similar levels but different mRNA, find that preferential association large polysomes less RNA. Third, translation synthetic cell-free extracts inhibited 3'UTR. Fourth, 3'UTR, when cis, can repress a heterologous transcript. These observations raise possibility may regulated vivo binding factors acting on