Utilizing Fibrin-Alginate and Matrigel-Alginate for Mouse Follicle Development in Three-Dimensional Culture Systems.

摘要: In vitro culture of ovarian follicles is a new technique in reproductive technology, which helps understanding the process folliculogenesis. The could be carried out using three-dimensional (3D) natural scaffolds that mimic tissue stroma. Selection right matrix and media these increase survival maturation follicles. this work, applicability matrigel-alginate (MA) fibrin-alginate (FA) 3D for folliculogenesis was assessed. ovaries 13-day-old Naval Medical Research Institute (NMRI) mice were isolated distributed into control vitrification groups. Preantral (mean diameter: 120-140 μm) mechanically from vitrified-warmed ovaries, encapsulated MA or FA scaffold cultured 12 days. Follicle survival, growth, maturation, quantitative expression oocyte genes (Gdf9, Bmp15, Fgf8, KitL, Kit, Amh) proteins (GDF9 BMP15) Survival rate preantral groups found to significantly higher than vitrified Antrum formation similar all diameters increased during period. A decreasing pattern gene seen This trend verified through evaluation protein expression, there strong staining antral last day culture. better compared indicates matrix, being rich extracellular components, condition presents good environment follicle development.