DNA cloning by homologous recombination in Escherichia coli.

作者: Youming Zhang , Joep P.P. Muyrers , Giuseppe Testa , A. Francis Stewart

DOI: 10.1038/82449

关键词:

摘要: … 12), we observed that ET recombination efficiencies improve significantly when the … likely to improve on this efficient outcome. Here we show that ET recombination can be applied to two …

参考文章(20)
Joep P. P. Muyrers, Youming Zhang, A. Francis Stewart, ET-Cloning: Think Recombination First Genetic engineering. ,vol. 22, pp. 77- 98 ,(2000) , 10.1007/978-1-4615-4199-8_6
Vladimir Larionov, None, Direct isolation of specific chromosomal regions and entire genes by TAR cloning. Genetic engineering. ,vol. 21, pp. 37- 55 ,(1999) , 10.1007/978-1-4615-4707-5_3
Cooduvalli S Shashikant, Janet L. Carr, Jaya Bhargava, Kevin L Bentley, Frank H Ruddle, Recombinogenic targeting: a new approach to genomic analysis—a review Gene. ,vol. 223, pp. 9- 20 ,(1998) , 10.1016/S0378-1119(98)00369-2
Youming Zhang, Frank Buchholz, Joep P.P. Muyrers, A. Francis Stewart, A new logic for DNA engineering using recombination in Escherichia coli Nature Genetics. ,vol. 20, pp. 123- 128 ,(1998) , 10.1038/2417
F Buchholz, J P Muyrers, A F Stewart, Y Zhang, RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners. Genes & Development. ,vol. 14, pp. 1971- 1982 ,(2000) , 10.1101/GAD.14.15.1971
Joep PP Muyrers, Youming Zhang, Giuseppe Testa, A Francis Stewart, Rapid modification of bacterial artificial chromosomes by ET-recombination. Nucleic Acids Research. ,vol. 27, pp. 1555- 1557 ,(1999) , 10.1093/NAR/27.6.1555
Kenan C. Murphy, Kenneth G. Campellone, Anthony R. Poteete, PCR-mediated gene replacement in Escherichia coli Gene. ,vol. 246, pp. 321- 330 ,(2000) , 10.1016/S0378-1119(00)00071-8
P.-O. Angrand, N. Daigle, F. van der Hoeven, H. R. Scholer, A. F. Stewart, Simplified generation of targeting constructs using ET recombination. Nucleic Acids Research. ,vol. 27, ,(1999) , 10.1093/NAR/27.17.E16-I