Development and characterization of nine polymorphic microsatellite markers for Mexican spadefoot toads (Spea multiplicata) with cross-amplification in Plains spadefoot toads (S-bombifrons)
作者： T. BECKER , R. A. NEWMAN , C. LEBONVILLE , G. R. HARPER , K. S. PFENNIG
DOI: 10.1111/J.1755-0998.2008.02291.X
关键词:
摘要: We developed nine polymorphic microsatellite markers for the Mexican spadefoot toad, Spea multiplicata. Allele numbers range from five to 12, with observed heterozygosities 0.48 0.87. Because two loci are in linkage disequilibrium, these provide eight independent markers. Three exhibit departure Hardy-Weinberg equilibrium, possibly resulting null alleles or population admixture. These will be useful assessing structure and relatedness S. Based on our success at cross-amplification Plains toad (Spea bombifrons), also may this species additional optimization.
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