Long-lasting effects of antibiotics on bacterial communities of adult flies.

摘要: Insect symbionts benefit their host and study requires large spectrum antibiotic use like tetracycline to weaken or suppress symbiotic communities. While antibiotics have a negative impact on insect fitness, little is known about effects microbial communities how long they last. We characterized the bacterial of adult cabbage root fly Delia radicum in Wolbachia-free population evaluated effect treatment these over several generations. Three D. generations were used: first- second-generation flies either ingested not, while third-generation untreated but differed with parents and/or grandparents that had not been treated. Fly sequenced using 16S rRNA gene. Tetracycline decreased diversity induced modifications both abundance relative frequencies, still visible offspring whose treated, therefore demonstrating long-lasting transgenerational animal microbiomes after treatment. Flies an history shared genera, potentially resistant heritable. Next, transmission should be investigated by comparing development stages plant compartments assess vertical horizontal transmissions