Sodium hydroxide-induced conformational change in schizophyllan detected by the fluorescence dye, aniline blue

摘要: Molecular conformation is considered to be an important factor in determining the biological activity of glucans; however, a simple method detect change for glucans solution has not been developed. We found that fluorescence intensity aniline blue bound schizophyllan (SPG) can used estimate relative amount single helix converting triple during different stages denature–renature cycle. This observation provides monitor conformational simpler and easier perform than other techniques (such as solid-state 13C NMR spectroscopy). The native SPG [a branched β-(1→3) glucan] rigid, closed helix. Treatment with NaOH, followed by neutralization, produces helix-rich preparation. observed does stain SPG, but will renatured NaOH-treated SPG. suggests binds only forms Further supporting evidence decreased on consecutive days after which consistent report gradually lost 77% their component 1 week (N. Nagi, N. Ohno, Y. Adachi, J. Aketagawa, H. Tamura, Shibata, S. Tanaka, T. Yadomae, Biol. Pharm. Bull., 16 (1993) 822–828). activates Limulus amebocyte lysate (LAL). reactivity stabilized at days, was evaluated using glucan sensitive LAL. LAL toward over time, suggesting detected correlated activity.