The SNP polymorphisms associated with WSSV-resistance of prophenoloxidase in red swamp crayfish (Procambarus clarkii) and its immune response against white spot syndrome virus (WSSV)
作者： Long Zhang , Yanhe Li , Minglin Wu , Haofeng Ouyang , Ruixue Shi
DOI: 10.1016/J.AQUACULTURE.2020.735787
关键词:
摘要: Abstract Prophenoloxidase (proPO), a critical enzyme in innate immune, has been extensively studied various crustacean species including red swamp crayfish Procambarus clarkii. However, little is known about the genetic structure of proPO from P. clarkii and its role antivirals. In this study, expression pattern upon white spot syndrome virus (WSSV) infection was investigated, genomic DNA sequence obtained for first time analyzed. The results indicated that mRNA level could be significantly upregulated haemocytes, hepatopancreas, gill midgut. 9085 bp length, which includes five exons interrupted by four introns, an 82 bp 5′-flanking region, 1671 bp 3′-untranslated region. Two TATA boxes as well three repeat sequences were predicted intron region proPO. Subsequently, single nucleotide polymorphisms (SNPs) identified genotyped population association between SNPs anti-WSSV traits investigated. Three regions identified, SNP 7081 T > C showed significant (P
sci-hub.st 参考文章(56)
Zhiqiang Xu, Liuxiu Tang, Yuehua Li, Jiachun Ge, Jianlin Pan, Identification of SNPs in the 5′-flanking region and 3′-UTR of the MIH gene and their association with precocity of the Chinese mitten crab Eriocheir sinensis Aquaculture Research. ,vol. 47, pp. 992- 1000 ,(2016) , 10.1111/ARE.12559
Nguyen Minh Thanh, Andrew C. Barnes, Peter B. Mather, Yutao Li, Russell E. Lyons, Single nucleotide polymorphisms in the actin and crustacean hyperglycemic hormone genes and their correlation with individual growth performance in giant freshwater prawn Macrobrachium rosenbergii Aquaculture. ,vol. 301, pp. 7- 15 ,(2010) , 10.1016/J.AQUACULTURE.2010.02.001
Xiaolian Liu, Weimin Wang, Yanhe Li, Yasmeen Gul, Wei Luo, Jie Zhang, DNA extraction from crayfish exoskeleton. Indian Journal of Experimental Biology. ,vol. 49, pp. 953- 957 ,(2011)
Weiqing Wang, The Molecular Detection of Corynespora Cassiicola on Cucumber by PCR Assay Using DNAman Software and NCBI international conference on computer and computing technologies in agriculture. pp. 248- 258 ,(2015) , 10.1007/978-3-319-48354-2_26
Yingying Wu, Xianhong Meng, Jie Kong, Sheng Luan, Kun Luo, Qingyin Wang, Yongyun Zheng, Screening white spot syndrome virus (WSSV)-resistant molecular markers from Fenneropenaeus chinensis Journal of Ocean University of China. ,vol. 16, pp. 129- 136 ,(2017) , 10.1007/S11802-017-3136-8
Jie Yang, Xuan Liu, Dan Wang, Chao Ning, Haifei Wang, Qin Zhang, Li Jiang, Functional validation of GPIHBP1 and identification of a functional mutation in GPIHBP1 for milk fat traits in dairy cattle. Scientific Reports. ,vol. 7, pp. 8546- 8546 ,(2017) , 10.1038/S41598-017-08668-6
Shaokui Yi, Yanhe Li, Linlin Shi, Long Zhang, Novel Insights into Antiviral Gene Regulation of Red Swamp Crayfish, Procambarus clarkii, Infected with White Spot Syndrome Virus Genes. ,vol. 8, pp. 320- ,(2017) , 10.3390/GENES8110320
Xin Zhang, Danping Huang, Xiwei Jia, Zhihua Zou, Yilei Wang, Ziping Zhang, Functional analysis of the promoter of the molt-inhibiting hormone (mih) gene in mud crab Scylla paramamosain. General and Comparative Endocrinology. ,vol. 259, pp. 131- 140 ,(2017) , 10.1016/J.YGCEN.2017.11.014
Pattamaporn Kwankaew, Rachanida Praparatana, Phanthipha Runsaeng, Prapaporn Utarabhand, An alternative function of C-type lectin comprising low-density lipoprotein receptor domain from Fenneropenaeus merguiensis to act as a binding receptor for viral protein and vitellogenin. Fish & Shellfish Immunology. ,vol. 74, pp. 295- 308 ,(2018) , 10.1016/J.FSI.2017.12.044
Yang Yang, Zhaojun Lan, Hu Shu, Huiqiang Zhou, Xiaolu Jiang, Liping Hou, Pinghua Gu, Association between expression levels and growth trait-related SNPs located in promoters of the MC4R and MSTN genes in Spinibarbus hollandi Genes & Genomics. ,vol. 40, pp. 1119- 1125 ,(2018) , 10.1007/S13258-018-0666-4