Lamin is essential for nuclear localization of the GPI synthesis enzyme PIG-B and GPI-AP production in Drosophila.
作者： Kazuhiro Furukawa , Satoshi Goto , Miki Yamamoto-Hino , Kohei Kawaguchi , Masaya Ono
DOI: 10.1242/JCS.238527
关键词:
摘要: Membrane lipid biosynthesis is a complex process that occurs in various intracellular compartments. In Drosophila, phosphatidylinositol glycan (PIG)-B (DPIG-B), which catalyzes addition of the third mannose glycosylphosphatidylinositol (GPI), localizes to nuclear envelope (NE). Although this NE localization essential for Drosophila development, underlying molecular mechanism remains unknown. To elucidate mechanism, we identified DPIG-B-interacting proteins by performing immunoprecipitation followed proteomic analysis. We then examined these are required DPIG-B. Knockdown Lamin Dm0, B-type lamin, led mislocalization DPIG-B from endoplasmic reticulum. Dm0 associated with at inner membrane, tail domain Dm0. Furthermore, GPI moieties were distributed abnormally mutant. These data indicate involved and proper GPI-anchor modification proteins.
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