Comparative proteomics and codon substitution analysis reveal mechanisms of differential resistance to hypoxia in congeneric snails.

摘要: Abstract Although high-throughput proteomics has been widely applied to study mechanisms of environmental adaptation, the conclusions from studies that are based on one species can be confounded by phylogeny. We compare freshwater snail Pomacea canaliculata (a notorious invasive species) and its congener diffusa non-invasive understand molecular their differential resistance hypoxia. A 72-h acute exposure experiment showed P. is more tolerant hypoxia than diffusa. The two were then exposed three levels dissolved oxygen (6.7, 2.0 1.0 mg L− 1) for 8 h, gill proteins analyzed using iTRAQ-coupled LC-MS/MS. striking differences in protein expression profiles, with having up-regulated signal transduction down-regulated glycolysis tricarboxylic acid cycle. Evolutionary analysis revealed five orthologous genes encoding differentially expressed clear positive selection, indicating selection acted some responsive genes. Our case highlighted potential integrated comparative evolutionary understanding genetic basis adaptation global change non-model species. Significance Rapid globalization recent decades greatly facilitated introduction around world. Successfully established introduced species, so-called have threatened invaded ecosystems. There substantial interest studying how respond extreme conditions because results help not only predict range expansion manage impact, but also may reveal adaptive underlying invasiveness. adopted a approach physiological proteomic responses congeneric snails various hypoxic conditions, as well codon substitution at transcriptomic level detect signals hypoxia-responsive physiological, other change.