Accurate genotyping of hepatitis C virus through nucleotide sequencing and identification of new HCV subtypes in China population

摘要: Nucleotide sequencing of the phylogenetically informative region NS5B remains gold standard for hepatitis C virus (HCV) genotyping. Here we developed a new methodology regions to increase accuracy and sensitivity HCV genotyping subtyping. The eight primers were identified by scanning full-length from 1127 genomic sequences found in databases. ability each pair amplify subtypes was scored, able better than previously used primers, therefore more accurately subtyping strains. Sequencing DNA amplified primer pairs can specifically correctly detect five (1a, 2a, 3b, 6a 1b). We further examined patient samples that identify clinical with high sensitivity. This method all 567 samples. Importantly, three novel (1b-2a, 1b-2k 6d-6k) samples, which have not been previous reported China. In conclusion, is reliable sensitive diagnostic tool using described could subtypes. Our research useful diagnosis, guidance treatment, management patients, studies on epidemiology HCV.