Description of bovine major histocompatibility complex class IIa haplotypes using parthenogenetic embryo-derived cells.

作者: A. L. A. Sá , V. S. G. Leal , M. P. C. Schneider , O. M. Ohashi , E. J. M. Santos

DOI: 10.1111/AGE.12285

关键词:

摘要: In this study, we report an approach to characterize individual BoLA haplotypes using cells from parthenogenetic bovine embryos derived slaughterhouse ovaries. Eight of the 15 so obtained had not undergone meiotic recombination on region and were suitable describe haplotypes. Detailed analysis class IIa identified seven different haplotypes, including six previously described two new alleles BoLA-DQA one BoLA-DQB. Our method provided reliable sources homozygous DNA

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