Chemical interactions of mercury species and some transition and noble metals towards metallothionein (Zn7MT-2) evaluated using SEC/ICP-MS, RP-HPLC/ESI-MS and MALDI-TOF-MS
作者： Ming Xu , Limin Yang , Qiuquan Wang
DOI: 10.1039/C3MT00016H
关键词:
摘要: The chemical affinities of three submetallomes (“Mn, Co, Ni, Cu, Cd”; “Pd, Pt, Au”; and “Hg, CH3Hg, C2H5Hg-THI”) towards metallothionein-2 (Zn7MT-2) in a physiological solution environment were evaluated using SEC/ICP-MS together with RP-HPLC/ESI-IT-MS MALDI-TOF-MS, followed the order: “Zn2+ Ni2+ > Mn2+, Co2+”; “Pd2+ Au3+ Pt2+”; “Hg2+ CH3Hg+ C2H5Hg-THI”. Besides these, structural change composition CH3Hg–MT-2 complexes formed during titration process further investigated CD spectroscopy, RP-HPLC/ESI-MS indicating that linear more hydrophobic (CH3Hg)xMT-2 (x = 12, 13, 14, 15, 16, 17, 18, 19 20) formed. This information is important understanding interactions metals Zn7MT-2 their corresponding biological functions toxicities.
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