Detection of a rare point mutation in Ki-ras of a human bladder cancer xenograft by polymerase chain reaction and direct sequencing.

摘要: This paper represents the first report of a codon 59 mutation in Ki-ras from spontaneous human transitional cell carcinoma bladder. Point mutations have potential to activate ras genes if they occur critical coding regions. These include sequences codons 12, 13, 59, 61 and 63. Mutations 13 been reported wide variety cancers, including However only retroviral as result vitro mutagenesis experiments. We used polymerase chain reaction direct sequencing detect Ki-ras, allele-specific restriction analysis N-ras xenografts continuous lines established bladder cancer biopsies ten different patients well biopsy specimens five tumours. For studies 139 bp fragment which spanned 12 128 that 63 were enzymatically amplified then sequenced. No detected. A heterozygous at GCA → G/ACA was detected one line. is being expressed appears stable it over several xenograft passages present paraffin-embedded tissue primary tumour patient. The biological significance currently under study.