Surface-catalyzed SAS-6 self-assembly directs centriole formation through kinetic and structural mechanisms

摘要: Discovering the physical principles directing organelle assembly is a fundamental pursuit in biology. Centrioles are evolutionarily conserved organelles with 9-fold rotational symmetry of chiral microtubules imparted onto cilia they template1. Centriole assemble from likewise symmetrical ring polymers SAS-6 proteins, orthogonal to toroidal surface surrounding resident centriole2-4. How properties ensure proper and arrangement not known. Here, we deployed photothermally-actuated off-resonance tapping high-speed atomic force microscopy (PORT-HS-AFM) decipher surface-guided self-assembly. Using machine learning quantify polymerization reaction developing coagulation-fragmentation model, discovered that shifts equilibrium by [~]104 compared solution situation, explaining emergence. Moreover, molecular dynamics PORT-HS-AFM revealed converts helical into residual asymmetry, which may impart features centrioles cilia. Overall, two robust centriole assembly.