Differentiation of Human-Induced Pluripotent Stem Cells Into Insulin-Producing Clusters by MicroRNA-7.

摘要: OBJECTIVES Diabetes results from inadequate insulin production pancreatic β-cells. Islet cell replacement is an effective approach for diabetes treatment; however, it not sufficient all diabetic patients. Thus, finding a new source with maturation of β-cells the major goal many studies. MicroRNAs are class small noncoding ribonucleic acid that regulate gene expression through posttranscriptional mechanisms. MicroRNA-7 has high level during islet development in humans, thereby playing critical role β-cell function. We study aimed to develop protocol differentiate human-induced pluripotent stem cells efficiently into isletlike clusters vitro by using microRNA-7. MATERIALS AND METHODS Human-induced colonies were transfected hsa-microRNA-7 siPORT NeoFX transfection agent. Total was extracted 24 and 48 hours after transfection. The transcription factors which important pancreases also performed. On third day, potency assessed response glucose levels. Diphenylthiocarbazone used identify existence presence Neurogenin-3 proteins investigated immunocytochemistry. RESULTS Morphologic changes observed on first day chemical transfection, formed day. specific increased significantly second positive stained secreted challenge test. CONCLUSIONS factor network endocrine differentiation. Chemical microRNA-7 can human induced functional short time.