Competitive exclusion of epiphytic bacteria by ice − Pseudomonas syringae mutants

摘要: The growth of ice nucleation-active and near-isogenic nucleation-deficient (Ice−) Pseudomonas syringae strains coexisting on leaf surfaces was examined to determine whether competition sufficient account for antagonism phylloplane bacteria. nucleation frequency spectra 46 Ice−P. mutants, obtained after mutagenesis with ethyl methanesulfonate, differed both quantitatively qualitatively, but the mutants could be grouped into four distinct phenotypic classes. numbers bacteria nuclei active at −5°C were reduced plants colonized mutant before challenge inoculations an Ice+P. wild-type strain. Frost injury pretreated also significantly compared that control correlated population size strain −5°C. An leaves, flowers, young fruit pears in field experiments colonization these tissues by Erwinia amylovora as untreated trees.