Alterations of p73 preferentially occur in gastric adenocarcinomas with foveolar epithelial phenotype.
作者： Hiroshi Yokozaki , Yoshinori Shitara , Jyun-ya Fujimoto , Toru Hiyama , Wataru Yasui
DOI: 10.1002/(SICI)1097-0215(19991008)83:2<192::AID-IJC8>3.0.CO;2-E
关键词:
摘要: To establish the possible involvement of p73, a newly discovered p53-related candidate as tumor-suppressor gene in human stomach carcinogenesis, allelic status, allelespecific expression and mutations were investigated using PCR‐restriction fragment length polymorphism (PCR-RFLP) analysis, RT-PCR SSCP analysis direct DNA sequencing 95 gastric adenocarcinomas. Of these, 32 exhibited heterozygous p73 allele for StyI restriction site exon 2. Among cancer 12 revealed loss heterozygosity (LOH) p73. All cancers with LOH phenotypes foveolar epithelium stomach. cases demonstrated not only bi-allelic but also relatively reduced affected 6 8 tumors LOH. No mutation was detected remaining LOH-positive cancers. Our results suggest that alterations including abnormal expression, may play roles genesis foveolar-type adenocarcinomas, though this is line classical Knudson’s ‘‘2-hit’’ model. Int. J. Cancer 83:192‐196, 1999. r 1999 Wiley-Liss, Inc.
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