Rapid, high-throughput detection of PrPSc by 96-well immunoassay.
作者： Michelle L. Kramer , Jason C. Bartz
DOI: 10.4161/PRI.3.1.8442
关键词:
摘要: Prion diseases are emerging infectious disorders that affect several mammalian species including humans. The transmissible agent is comprised of PrP(Sc), a misfolded isoform the normal host-encoded prion protein PrP(C). Immunodetection PrP(Sc) often utilized for disease diagnosis and tracking spread through host. We have developed rapid, high-throughput 96-well immunoassay, which specific detection PrP(Sc). This assay has limits similar to western blot advantageous because its comparatively shorter running time, smaller start-up operation costs large sample capacity.
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