Cyclooxygenase-2 Activity Altered the Cell-Surface Carbohydrate Antigens on Colon Cancer Cells and Enhanced Liver Metastasis

摘要: Cyclooxygenase-2 (COX-2) was recently reported (M. Tsujii and R. N. DuBois, Cell, 83: 493–501, 1995) to affect the metastatic potential of cells. Previous studies Fukuda, Cancer Res., 56: 2237–2244, 1996) indicated that sialyl Lewis antigen expression is correlated with hematogenous metastasis colon cancer. In present study, we investigated interaction between COX-2 activity, antigens, in vitro cancer cell adhesion endothelial cells, vivo potential. Effects activity prostaglandin E2 on adhesion, glycosyltransferase genes were determined Caco-2-m (COX-2 low level), Caco-2-COX-2 (programmed overexpress COX-2), HT-29 high level) Metastatic spread these cells liver also investigated. had increased SPan-1 levels adherence via compared expressed a adhered a. Treatment inhibitor, celecoxib, decreased β3Gal-T5 ST3Gal III IV inhibited by celecoxib enhanced treatment. metastasized liver, whereas did not. Pretreatment reduced as well anti-sialyl antibodies. Our results indicate direct link carcinoma accelerated production antigens. inhibitors may suppress metastasis.