Temporal and spatial expression patterns of TUB9, a β-tubulin gene of Arabidopsis thaliana
作者： Zigang Cheng , D. Peter Snustad , John V. Carter
关键词:
摘要: Transgenic plants carrying chimeric genes composed of segments the 5′-flanking region Arabidopsis 9-tubulin gene (TUB9) fused to coding β-glucuronidase (GUS) Escherichia coli were used investigate temporal and spatial patterns TUB9 expression. Chimeric that contained at least 800 bp DNA expressed primarily in floral tissues, with high levels expression observed pollen, elongating pollen tubes ovules. The reporter ovules ceased time fertilization. In situ hybridization was verify transgenic is representative endogenous gene. also provided new insight into transcript accumulation possible role functional implication largely non-overlapping tubulin are discussed.
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