Development of transgenic yellow poplar for mercury phytoremediation.

摘要: We examined the ability of yellow poplar ( Liriodendron tulipifera ) tissue cultures and plantlets to express modified mercuric reductase merA gene constructs. Mercury-resistant bacteria convert highly toxic, ionic mercury, Hg(ll), much less elemental Hg(O). Expression in transgenic plants might provide an ecologically compatible approach for remediation mercury pollution. Because alteration bacterial sequence is necessary high-level expression Arabidopsis thaliana , proembryogenic masses (PEMs) were transformed with three constructs via microprojectile bombardment. Each construct was synthesized have altered flanking regions increasing amounts coding sequence. All conferred resistance independently PEM colonies. Stability transgene increased parallel extent modification. Regenerated containing most merA merA18 germinated grew vigorously media normally toxic levels mercury. The released at approximately 10 times rate untransformed plantlets. These results indicate that expressing may a means phytoremediation