Laser Capture Microdissection for Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

摘要: Macrophage foam cells are critical mediators in atherosclerosis plaque development. A better understanding of the vivo transcript profile during formation and progression lesions may lead to novel therapeutic interventions. Toward this goal, we demonstrate for first time that cell-specific RNA can be purified from atherosclerotic arteries, a tissue mixed cellular composition. Foam apolipoprotein (apo) E-/- mice were isolated by laser capture microdissection (LCM); was extracted used molecular analysis real-time quantitative polymerase chain reaction. Compared whole tissue, significant enrichment transcripts achieved. Furthermore, test ability quantify differences gene expression response an inflammatory stimulus, apoE-/- injected with lipopolysaccharide, after which transcriptional induction mediators, VCAM, ICAM, MCP-1, observed lesional macrophage cell RNA. These approaches will facilitate study under various conditions formation, regression, genetic environmental perturbations.