Engineering of an oleate hydratase for efficient C10-Functionalization of oleic acid.
作者： Qi-Fan Sun , Yu-Cong Zheng , Qi Chen , Jian-He Xu , Jiang Pan
DOI: 10.1016/J.BBRC.2020.12.039
关键词:
摘要: Abstract Oleate hydratase catalyzes the hydration of unsaturated fatty acids, giving access to C10-functionalization oleic acid. The resultant 10-hydroxystearic acid is a key material for synthesis many biomass-derived value-added products. Herein, we report engineering an oleate from Paracoccus aminophilus (PaOH) with significantly improved catalytic efficiency (from 33 s−1 mM−1 119 s−1 mM−1), as well 3.4 times increased half-life at 30 °C. structural mechanism regarding impact mutations on activity and thermostability was elucidated aid molecular dynamics simulation. practical feasibility engineered PaOH variant F233L/F122L/T15 N demonstrated through pilot 10-oxostearic via optimized multi-enzymatic cascade reaction, space-time yields 540 g L−1 day−1 160 g L−1 day−1, respectively.
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