Design, expression, and evaluation of novel multiepitope chimeric antigen of Wuchereria bancrofti for the diagnosis of lymphatic filariasis - A structure-based strategy.

摘要: Abstract The Global Program for Elimination Lymphatic Filariasis (GPELF) is in an advanced stage and requires tools diagnosing infection, assessing transmission certification. This study was aimed at developing antibody-based assay using a chiemric antigen containing multi-B-cell epitopes from antigens highly expressed different stages of Wuchereria bancrofti to detect LF infection its transmission. express cloned two indirect ELISA based (IgG1 & IgG4 based) antibody assays were developed the recombinant antigen. chimeric displayed 1 3-fold reactivity with IgG1 antibodies, respectively microfilaraial (mf) positive sera when compared that samples Non-endemic normal (NEN) (O.D, 0.13 ± 0.20 0.18 ± 0.07), thus differentiating infected uninfected individuals. In assays, multiepitope also showed 0.27 ± 0.18 0.16 ± 0.03) small proportion (18 30, out 156) endemic individuals few (4) chronic patients (CP). did not react or antibodies malaria, scrub typhus, dengue, hookworm, roundworm helminth cases (0.139 ± 0.018, 0.144 ± 0.007 0.17804 ± 0.007 0.162 ± 0.006), showing high specificity. sensitivity (%) specificity multi-epitope antigen-based are 100, 98.1 99.52, respectively. Thus, appears have good potential detecting active communities.