The Mechanical and Biological Properties of Chitosan Scaffolds for Tissue Regeneration Templates Are Significantly Enhanced by Chitosan from Gongronella butleri

摘要: Chitosan with a molecular weight (MW) of 104 Da and 13% degree acetylation (DA) was extracted from the mycelia fungus Gongronella butleri USDB 0201 grown in solid substrate fermentation used to prepare scaffolds by freeze-drying method. The mechanical biological properties fungal chitosan were evaluated compared those prepared using chitosans obtained shrimp crab shells squid bone plates (MW 105-106 DA 10-20%). Under scanning electron microscopy, it observed that all had average pore sizes approximately 60-90 mm diameter. Elongated pores polygonal found crab, scaffolds. physico-chemical an effect on formation scaffolds, consequently influenced Fungal showed excellent mechanical, water absorption lysozyme degradation properties, whereas 106Da 12%) exhibited lowest rate. In evaluation biocompatibility ability fibroblast NIH/3T3 cells attach similar, but proliferation morphology faster than Therefore scaffold, which has is most suitable scaffold use as template for tissue regeneration.