Preparation, characterization, biotoxicity, and biodistribution of thermo-responsive magnetic complex micelles formed by Mn0.6Zn0.4Fe2O4 and a PCL/PEG analogue copolymer for controlled drug delivery

摘要: A thermo-responsive PCL/PEG analogue copolymer (PCL-[b-P(MEO2MA-co-OEGMA)]2) with a lower critical solution temperature (LCST) of 40.4 °C at an MEO2MA/OEGMA molar ratio 87 : 13 was designed and synthesized. The subsequently labeled by coupling fluorescein isothiocyanate (FITC). Thermo-responsive magnetic PCL-[b-P(MEO2MA-co-OEGMA)]2/Mn0.6Zn0.4Fe2O4 (MZF) complex micelles were prepared self-assembly method. Doxorubicin (DOX) loaded into the as model drug, DOX-MZF-micelles showed well-controlled release both externally fixed temperatures in presence alternating field (AMF). Both blank polymer exhibited excellent stability normal saline serum. Based on detection FITC fluorescence signal, found to be effectively FITC. Furthermore, biological toxicity studied vitro vivo. In studies evaluate cell viability performed employing WST-1 LDH assays using HL7702 cells, respectively. vivo biotoxicity conducted ICR mice through series tests: general conditions, body weight shifts, serum biochemistry profiles, organ coefficient tests. All results obtained from indicated their good biocompatibility nontoxicity. biodistribution FITC-labeled mice. cleared kidney spleen, while MZF nanoparticles liver time, causing no adverse effects organisms. shown ideal nanocarrier for anticancer drug delivery terms controlled release, stability, safety.