Coral-Associated Bacterial Diversity Is Conserved across Two Deep-Sea Anthothela Species

摘要: Cold-water corals, similar to tropical contain diverse and complex microbial assemblages. These bacteria provide essential biological functions within coral holobionts, facilitating increased nutrient utilization production of antimicrobial compounds. To date, few cold-water octocoral species have been analyzed explore the diversity abundance their associates. For this study, 23 samples family Anthothelidae were collected from Norfolk (n = 12) Baltimore Canyons 11) western Atlantic in August 2012 May 2013. Genetic testing found that these comprised two Anthothela (Anthothela grandiflora sp.) Alcyonium grandiflorum. DNA was extracted sequenced with primers targeting V4-V5 variable region 16S rRNA gene using 454 pyrosequencing GS FLX Titanium chemistry. Results demonstrated host primary driver bacterial community composition. Al. grandiflorum, dominated by Alteromonadales Pirellulales had much higher richness, a distinct compared samples. (A. very communities, Oceanospirillales Spirochaetes. Additional analysis core-conserved at 90% sample coverage revealed genus level conservation across This core included unclassified Oceanospirillales, Kiloniellales, Campylobacterales, Spirochaeta. Members previously recognized for functional capabilities nitrogen cycling suggest possibility nearly complete cycle species. Overall, many associates identified study potential contribute acquisition nutrients holobiont.