Histochemical and topo-optical investigations on tissue-isolated and in vitro amyloid fibrils.

摘要: An improved microscopic slide preparation for staining of tissue-isolated and in vitro amyloid fibrils is described detail. Specimens were prepared by slow drying an fibril suspension on aminoalkyl-silanised glass slides underneath a coverglass. The purpose this method to obtain microscopically visible fibers. Polarization microscopy was performed unstained fibers different embedding media, stained with Congo red, toluidine blue, 1,9-dimethylmethylene anisotropic PAS reaction Sudan black. Results identical 8 preparations. refractive index about 1.5, as expected protein fibrils. red-stained showed yellow or blue–green polarization colors, while blue induced green orange colors. latter dyes the even buffers low pH high ionic strength, pointing strong acidic groups like sulphates amy...