Adenovirus targeted to heparan-containing receptors increases its gene delivery efficiency to multiple cell types

摘要: Adenovirus (Ad) is used as a vector for gene delivery in therapies involving genetic disease, vascular and cancer. The first step efficient transfer effective virus binding to the target cells. We have found that Ad-mediated multiple cell types much less compared epithelial-derived Low nonepithelial was directly correlated deficiency of cellular receptor which mediates Ad binding. To overcome this inefficiency we constructed new virus, AdPK, contains heparin-binding domain targets broadly expressed, heparan-containing receptors. AdPK delivers genes at markedly higher efficiencies than unmodified Ad. Viruses with enhanced attachment characteristics significantly improve efficiency may expand tissues amenable therapy.