Local and systemic factors in periodontal disease increase matrix-degrading enzyme activities in rat gingiva: effect of micocycline therapy.

摘要: We previously reported that both local and systemic factors relevant to the pathogenesis of periodontal disease can increase gingival collagenase activity in rats. Since degradation extracellular matrix is an essential feature this tissue breakdown requires multiple enzyme interactions, current study was carried out determine effects bacterial endotoxin (LPS) (a factor) diabetes on a panel matrix-degrading enzymes (collagenase, gelatinase, elastase, beta-glucuronidase) gingiva In addition, therapy with semisynthetic tetracycline (minocycline) were investigated. Ten male, Sprague-Dawley rats made diabetic by IV injection streptozotocin. Four ten then received minocycline (10 mg/day) oral gavage daily basis for 3 weeks. Nineteen nondiabetic served as controls 9 them 10 microliters E. coli LPS mg/ml) into labial every other day during last week study. The sham injected saline gingiva. At end experimental period, skin dissected from each rat extracted analysis. Our results showed markedly increased four activities skin. contrast, these alone. Systemic completely ameliorated elevated levels Minocycline added vitro assay systems containing extract also inhibited gelatinase activities, but no inhibition observed elastase beta-glucuronidase indicating MMPs minocycline, diabetes, indirect mechanisms, respectively.