Age and gender effect on the growth of bone marrow stromal cells in vitro.

摘要: Abstract Bone marrow harvested by aspiration contains connective tissue progenitor cells which can be selectively isolated and induced to express bone phenotype in vitro. The osteoblastic estimated counting the number of attach using haemacytometer. This study was undertaken test hypothesis that human aging is associated with a significant change on progenitors marrow. aspirates were from 38 patients, 14 men (age 11-70) 24 women 10-70) cultured F12: DMEM (1:1). In total 15 samples have been patients above 40 years old (men/women) age. Fourteen (93.3%) this failed proliferate. Only one (6.7%) sample male patient, aged 59 successfully cultured. Seventy percent (16/23) patient below than However, our observation survival rate for different gender does not indicate any difference. From study, we conclude growth stromal possibly engineering better younger patient.