作者: Denis G. Baskin
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摘要: Before the middle of previous century, cell types pancreatic islets Langerhans were identified primarily on basis their color reactions with histological dyes. At that time, chemical for staining properties islet cells in relation to identity, chemistry and structure hormones was not fully understood. Nevertheless, definitive secrete glucagon, insulin, somatostatin (A, B, D cells, respectively) could reliably be differentiated from each other protocols involved variations one or more tinctorial techniques, such as Mallory-Heidenhain azan trichrome, chromium hematoxylin phloxine, aldehyde fuchsin, silver impregnation methods, which popularly used until supplanted by immunohistochemical techniques. antibody-based most bona fide histochemical techniques identification B based detection sulfhydryl disulfide groups insulin. The application classical methods pathophysiological studies physiological experiments fundamental our understanding architecture roles A glucose regulation diabetes.