Expression of a quail bHLH transcription factor is associated with adrenergic development in trunk neural crest cultures.
作者： Anne K. Hennig , Gerald D. Maxwell
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摘要: 1. Expression of the chick type 1 basic helix–loop–helix transcription factor GbHLH1.4 persists in several embryonic regions, including some where neural crest cells differentiate (Helms, J. A., et al., Mech. Dev.48:93–108, 1994.) We have cloned portions quail homologue (designated QbHLH) order to investigate its expression and possible function cultures. Three sets polymerase chain reaction primers were used amplify cDNA sequences encompassing much coding region outside bHLH domain. Two primer amplified a single band from all tissues tested. The third set produced two bands, differing by 72-base pair insertion, both which present assayed.
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