The growth factor-platelet-transformation connection.

摘要: Until recently, the growth of diploid animal cells in monolayer culture re quired that defined nutrient medium be supplemented with serum prepared from clotted whole blood (Temin et al., 1972; Holley, 1975). The concentration added to was found a major determinant rate and final population density these cultures (Holey, 1974). Recently, purification growth-promoting factors development chemically media able support some cell types without presence (reviewed by Barnes Sato, 1980) have supported view provides growth-stimulatory as well nutrients or vitamins, both.