Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation

摘要: Chronically haemodialysed end-stage renal disease patients are at high risk of morbidity arising from complications dialysis, the underlying pathology that has led to and complex chronic kidney disease. Anaemia is commonplace its origins multifactorial, involving reduced erythropoietin production, accumulation uremic toxins an increase in erythrocyte fragility. Oxidative damage a common factor co-morbidities known cause Therefore, we have investigated hypothesis specific membrane proteins more oxidised vitamin C supplementation can ameliorate protein oxidation. Eleven 15 control subjects were recruited study. Patients supplemented with 2 × 500 mg per day for 4 weeks. Erythrocyte prepared pre- post-vitamin determination Total carbonyls by but not dialysis when enzyme linked immunosorbent assay. Using western blot detect proteins, one band, later identified as containing ankyrin, was found be controls significantly 60% all after 20% treatment pre-dialysis. Ankyrin oxidation analysis may useful stratified medicines approach possible marker identify requirements intervention patients.